en Summer Savory (Satureja hortensis) Can Reduce Spermatotoxic Effects of Doxorubicin in Rats Assisted reproductive technologies (ART) Assisted reproductive technologies (ART) پژوهشي Original Research <p class="MsoNormal" style="margin: 0in 42.55pt 0.0001pt line-height: 14pt"><font face="arial, helvetica, sans-serif"><b><span style="font-size: 10pt">Introduction:</span></b><b><span style="font-size: 10pt color: rgb(0, 0, 153)">&nbsp;</span></b><span lang="EN-GB" style="font-size: 10pt">Application of doxorubicin (DOX), an anticancer anthracycline antibiotic, carries the risk of serious dose-dependent toxicity to non-target tissues. The aim of this study was to investigate whether DOX-induced spermatotoxicity could be prevented by using the Satureja hortensis hydroalcoholic extract (SE). </span><span lang="EN-GB" style="font-size: 10pt">&nbsp;</span><span style="font-size: 10pt"><o:p></o:p></span></font></p> <p class="MsoNormal" style="margin: 0in 42.55pt 0.0001pt line-height: 14pt"><font face="times new roman, times, serif"><b><span style="font-size: 10pt">Methods:</span><span style="font-size: 10pt color: rgb(0, 102, 153)">&nbsp;</span></b><span lang="EN-GB" style="font-size: 10pt">Male Wistar rats were randomly divided into four groups of six rats each. DOX was administered to two groups of rats in 5 equal intraperitoneal injections over a period of 4 weeks (accumulated dose of 20 mg/kg). One of these groups received SE at a dose of 100 mg/kg per day subcutaneously for 28 days along with DOX. A vehicle treated control group and a SE control group were also included.</span><span style="font-size: 10pt"><o:p></o:p></span></font></p> <p class="MsoNormal" style="margin: 0in 42.55pt 0.0001pt line-height: 14pt"><font face="times new roman, times, serif"><b><span style="font-size: 10pt">Results:</span></b><span style="font-size: 10pt">&nbsp;</span><span lang="EN-GB" style="font-size: 10pt">Epididymal sperm analyses revealed that DOX caused significant decreases in sperm concentration, viability and motility along with elevated sperm abnormality, while SE co-administration provided marked normalization in the sperm quantity and quality compared to the DOX-only treated group.</span><span lang="EN-GB" style="font-size: 10pt">&nbsp;</span><span style="font-size: 10pt"><o:p></o:p></span></font></p> <p class="MsoNormal" style="margin: 0in 42.55pt 0.0001pt line-height: 14pt"><font face="times new roman, times, serif"><b><span style="font-size: 10pt">Conclusion:</span></b><span style="font-size: 10pt">&nbsp;</span><span lang="EN-GB" style="font-size: 10pt">It is reasonable to suppose that protective effects of SE against DOX-induced reproductive toxicity might have been related to the antioxidative and anti-inflammatory effects of this substance.</span></font><b><span style="font-size: 10pt"><o:p></o:p></span></b></p> Doxorubicin, Rat, Satureja hortensis, Sperm 1 6 http://caspjrm.ir/browse.php?a_code=A-12-34-2&slc_lang=en&sid=1 Ali Shalizar Jalali ali_shalizar@yahoo.com 10031947532846001686 10031947532846001686 Yes Urmia University, Urmia, Iran Urmia University, Urmia, Iran Gholamreza Najafi 10031947532846001687 10031947532846001687 No Histology and Embryology Research Laboratories, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Urmia University, Urmia, Iran Parisa Rahimzadeh 10031947532846001688 10031947532846001688 No Graduated, MSc in Plant Physiology Urmia University, Urmia, Iran