en Embryology Embryology پژوهشي Original Research <p></p> <div data-url="http://caspjrm.ir/form_send_article.php" id="link64_adl_tabid" style="display:none;">178</div> <p style="margin-left:42.55pt;"><a name="OLE_LINK17"></a><a name="OLE_LINK16"><span style="color:#000000;"><strong>Background:</strong></span></a> The <a name="OLE_LINK268"></a><a name="OLE_LINK267">basic pathophysiologic phenomenon</a> in testicular torsion, a common urologic emergency, is ischemia followed by reperfusion. In this study, we evaluated the effect(s) of simvastatin (SIM), a lipid lowering agent with antioxidant and anti-inflammatory properties, on mouse epididymal sperm fertilizing potential and the subsequent in vitro embryo development in experimentally-induced unilateral testicular ischemia-reperfusion (IR).</p> <p style="margin-left:42.55pt;"><strong>Methods:</strong> Adult male mice <a name="OLE_LINK270"></a><a name="OLE_LINK269">were divided into four groups </a>(n = 6, each). Following anaesthesia, IR was induced by clamping the left testicular vessels with an atraumatic microvascular clamp for 30 minutes in the IR group. In IR+SIM group, in addition, the mice received SIM (20 mg/kg per day) orally for 3 days starting from the day of induction of the experimental IR. A vehicle-treated control group and a SIM-only treated group were also included. Ipsilateral and contralateral epididymal sperms fertilizing capacity was analyzed in four groups after 35 days.</p> <p style="margin-left:42.55pt;"><strong>Results:</strong> Significant reduction in fertilization as well as blastulation rates were observed in the IR group. However, the SIM treatment considerably attenuated the IR-induced <a name="OLE_LINK272"></a><a name="OLE_LINK271">negative alterations</a> in the above-mentioned parameters.</p> <p style="margin-left:42.55pt;"><strong>Conclusion:</strong> These findings revealed the repro-protective effects of SIM <a name="OLE_LINK273">on the murine model</a> of IR through the inhibition of oxidative injuries and inflammatory reactions.</p> Ischemia-reperfusion, Simvastatin, Fertilization, Blastocyst, Mice 10 14 http://caspjrm.ir/browse.php?a_code=A-12-34-3&slc_lang=en&sid=1 Ali Shalizar Jalali 10031947532846001662 10031947532846001662 Yes Dr. Ali Shalizar Jalali, Histology and Embryology Research Laboratories, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran, Telefax: +98-44-32771926, Email: ali_shalizar@yahoo.com Mehdi Behfar m.behfar@gmail.com 10031947532846001663 10031947532846001663 No Urmia University Gholamreza Najafi g.najafi@yahoo.com 10031947532846001664 10031947532846001664 No Urmia University Alireza Nourian norian.alireza@gmail.com 10031947532846001665 10031947532846001665 No Urmia University Mehdi Shahkarimi mehdikarimi1056@gmail.com 10031947532846001666 10031947532846001666 No Urmia University Mobin Koohestani 10031947532846001667 10031947532846001667 No Urmia University