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:: دوره 3، شماره 1 - ( 4-1396 ) ::
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Determination and comparison of the expression levels of various germ cell-specific genes in human bone marrow- and peripheral blood-derived mesenchymal stem cells
:   (1112 مشاهده)

Background: A number of reports have shown that mesenchymal stem cells (MSCs) from various sources, expressed a number of germ cell (GC)-specific genes innately but there wasn’t any complete and quantitative study. So that, in this study we determined and compared the expression levels of some famous GC-specific genes in human male bone marrow (BM)- and peripheral blood (PB)-MSCs.

Mehtods: Human BM- and PB-MSCs were isolated using a density gradient factor and centrifuging. Then, Passage 3 of both cell types were characterized by flow cytometric analysis and differentiation test and then evaluated by real time RT-PCR for the expression levels of GC-specific genes.

Results: Both of the cell types obviously expressed c-kit, Dazl, Fragilis, Itgb1, Nanos3, Oct4 and Blimp1 at high levels and except Itgb1 which was expressed at a higher level in PB-MSCs than in BM-MSCs (p<0.05), levels of expression for the other markers were almost similar in the two cell types. Different levels of Itgb1 expression may be related to their different niches. Furthermore, both of the cell types were negative for Fkbp6, Stra8, Scp3, Tex13 and Vasa and weakly expressed Stella. Moreover, BM-MSCs had low expression levels of Piwil2 and this marker wasn’t expressed in PB-MSCs. 

Conclusion: Overall, it could be said that BM- and PB-MSCs have a little bit different GC-specific gene expression pattern. Although, these data are very important, so many detailed and creative research studies still needed in order to find the way of using MSCs in cell-based therapies of infertility.

     
نوع مطالعه: پژوهشي | موضوع مقاله: Stem cells
دریافت: ۱۳۹۶/۱/۱۸ | پذیرش: ۱۳۹۶/۲/۲۸ | انتشار: ۱۳۹۶/۳/۶
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Esmaeilnejad Ganji S M, Ghasemzadeh-Hasankolaei M, Jorsaraei S G A, Esmaeilzadeh S. Determination and comparison of the expression levels of various germ cell-specific genes in human bone marrow- and peripheral blood-derived mesenchymal stem cells. Caspian J Reprod Med. 2017; 3 (1) :2-12
URL: http://caspjrm.ir/article-1-115-fa.html

Determination and comparison of the expression levels of various germ cell-specific genes in human bone marrow- and peripheral blood-derived mesenchymal stem cells. نشریه طب تولید مثل خزر. 1396; 3 (1) :2-12

URL: http://caspjrm.ir/article-1-115-fa.html



دوره 3، شماره 1 - ( 4-1396 ) برگشت به فهرست نسخه ها
Caspian Journal of Reproductive Medicine
Caspian J Repord Med: Infertility and Reproductive Health Research Center of Babol University of Medical Sciences, Babol-Amol old highway, after Mohammadhasan Khan bridge, Babol, Mazandaran, Iran, P.O. Box: 47135-547 Tel: +98-11-32274881-2 Fax: +98-11-32274880 E-mail: info@caspjrm.ir Website: www.caspjrm.ir
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